Reduction of NADH oxidase, NO synthase, TNFα, and IL-1β mRNA expression levels on lipopolysacharide-stimulated murine macrophages by Zataria Multiflora
نویسندگان
چکیده مقاله:
Zataria multiflora (ZM) is a thyme-like aromatic plant in the Lamiaceae family that grows in central and southern Iran. ZM is extensively used as a flavor ingredient in a wide variety of foods and is used as part of popular traditional folk remedies. In the present study, ZM essential oil (ZMO) was obtained from ZM leaves via hydro-distillation and then analyzed by GC-MS (gas chromatography-mass spectrometry). The anti-inflammatory activity of ZMO was determined via measures of NADH oxidase (NOX), inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α, and interleukin (IL)-1β mRNA expression in lipopolysaccharide-stimulated murine macrophages using real-time polymerase chain reaction (PCR). GC-MS analysis indicated that the main components in the ZMO were carvacrol (29.4%), thymol (25.7%), p-cymene (11.2%), linalool (9.3%), and γ-terpinene (8.0%). ZMO significantly reduced NOX, iNOS, TNFα, and IL-1β mRNA expression in cells at concentrations of 0.1-1 μg/mL, indicating a capacity for this product to potentially modulate/diminish immune responses. ZMO has anti-oxidant and anti-inflammatory properties and could be potentially used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and a number of inflammatory conditions associated with stress.
منابع مشابه
reduction of nadh oxidase, no synthase, tnfα, and il-1β mrna expression levels on lipopolysacharide-stimulated murine macrophages by zataria multiflora
zataria multiflora (zm) is a thyme-like aromatic plant in the lamiaceae family that grows in central and southern iran. zm is extensively used as a flavor ingredient in a wide variety of foods and is used as part of popular traditional folk remedies. in the present study, zm essential oil (zmo) was obtained from zm leaves via hydro-distillation and then analyzed by gc-ms (gas chromatography-mas...
متن کاملاثر اسانس آویشن شیرازی بر تولید یون سوپراکسید و نیتریک اکسید و بیان ژنهای NADH اکسیداز و نیتریک اکسید سنتاز در سلولهای ماکروفاژ
Objective: In this study, inhibitory effect of Zataria multiflora essential oil on superoxide and nitric oxide production and NADH oxidase (NOX) and nitric oxide synthase (NOS) expression was examined. Materials and Methods: Zataria essential oil was obtained by hydro-distillation and chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). Superoxide and ni...
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عنوان ژورنال
دوره 2 شماره 3
صفحات 89- 100
تاریخ انتشار 2013-07-01
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